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Target
Cathepsin B-like cysteine protease
Ligand
BDBM22069
Substrate
BDBM22047
Meas. Tech.
Enzyme Inhibition Assay
pH
5.5±n/a
Temperature
295.15±n/a K
IC50
>25000±n/a nM
Citation
 Mallari, JP; Shelat, AA; Obrien, T; Caffrey, CR; Kosinski, A; Connelly, M; Harbut, M; Greenbaum, D; McKerrow, JH; Guy, RK Development of potent purine-derived nitrile inhibitors of the trypanosomal protease TbcatB. J Med Chem 51:545-52 (2008) [PubMed]  Article 
Target
Name:
Cathepsin B-like cysteine protease
Synonyms:
CPC | Cathepsin B-Like Cysteine Protease (TbcatB) | Cysteine peptidase C | TbcatB
Type:
Enzyme
Mol. Mass.:
37220.37
Organism:
Trypanosoma brucei
Description:
Enzyme was expressed in yeast Pichia pastoris.
Residue:
340
Sequence:
MHLMRACITFCIASTAVVAVNAALVAEDAPVLSKAFVDRVNRLNRGIWKAKYDGVMQNITLREAKRLNGVIKKNNNASILPKRRFTEEEARAPLPSSFDSAEAWPNCPTIPQIADQSACGSCWAVAAASAMSDRFCTMGGVQDVHISAGDLLACCSDCGDGCNGGDPDRAWAYFSSTGLVSDYCQPYPFPHCSHHSKSKNGYPPCSQFNFDTPKCNYTCDDPTIPVVNYRSWTSYALQGEDDYMRELFFRGPFEVAFDVYEDFIAYNSGVYHHVSGQYLGGHAVRLVGWGTSNGVPYWKIANSWNTEWGMDGYFLIRRGSSECGIEDGGSAGIPLAPNTA
  
Inhibitor
Name:
BDBM22069
Synonyms:
6-{[(4-chlorophenyl)methyl]amino}-9-(cyclohexylmethyl)-9H-purine-2-carbonitrile | Compound 3{10,15}
Type:
Small organic molecule
Emp. Form.:
C20H21ClN6
Mol. Mass.:
380.874
SMILES:
Clc1ccc(CNc2nc(nc3n(CC4CCCCC4)cnc23)C#N)cc1
Structure:
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Substrate
Name:
BDBM22047
Synonyms:
CBZ-Phe-Arg 7-amido-4-methylcoumarin | Z-Phe-Arg-AMC
Type:
Fluorogenic substrate
Emp. Form.:
C33H36N6O6
Mol. Mass.:
612.6755
SMILES:
[#6]-c1cc(=O)oc2cc(-[#7]-[#6](=O)-[#6](-[#6]-[#6]-[#6]\[#7]=[#6](\[#7])-[#7])-[#7]-[#6](=O)-[#6](-[#6]-c3ccccc3)-[#7]-[#6](=O)-[#8]-[#6]-c3ccccc3)ccc12
Structure:
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