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TargetBeta-secretase 1
LigandBDBM25361
Substrate/CompetitorBACE Substrate Peptide
Meas. Tech.In Vitro BACE1 Activity Assay
pH4.5±n/a
Temperature295.15±n/a K
IC50 2800±120 nM
Citation Barrow, JCStauffer, SRRittle, KENgo, PLYang, ZSelnick, HGGraham, SLMunshi, SMcGaughey, GBHolloway, MKSimon, AJPrice, EASankaranarayanan, SColussi, DTugusheva, KLai, MTEspeseth, ASXu, MHuang, QWolfe, APietrak, BZuck, PLevorse, DAHazuda, DVacca, JP Discovery and X-ray crystallographic analysis of a spiropiperidine iminohydantoin inhibitor of beta-secretase. J Med Chem51:6259-62 (2008) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Beta-secretase 1
Name:Beta-secretase 1
Synonyms:ASP2 | Asp 2 | Aspartyl protease 2 | BACE | BACE1 | Beta-secretase (BACE) | Beta-secretase 1 | Beta-secretase 1 (BACE 1) | Beta-secretase 1 (BACE-1) | Beta-secretase 1 (BACE1) | Beta-site APP cleaving enzyme 1 | Beta-site amyloid precursor protein cleaving enzyme 1 | KIAA1149 | Memapsin-2 | Membrane-associated aspartic protease 2 | beta-Secretase (BACE-1) | beta-Secretase (BACE1)
Type:Protein
Mol. Mass.:55755.10
Organism:Homo sapiens (Human)
Description:P56817
Residue:501
Sequence:
MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSF
VEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSST
YRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGIL
GLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGI
DHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKK
VFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRIT
ILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSAC
HVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQW
RCLRCLRQQHDDFADDISLLK
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  Blast E-value cutoff:
BDBM25361
NameBDBM25361
Synonyms:N-(4-{[4-(cyclohexylamino)-1-(3-fluorophenyl)-2-oxo-1,3,8-triazaspiro[4.5]dec-3-en-8-yl]methyl}phenyl)acetamide | Spiropiperidine Iminohydantoin, 16
TypeSmall organic molecule
Emp. Form.C28H34FN5O2
Mol. Mass.491.6003
SMILESCC(=O)Nc1ccc(CN2CCC3(CC2)N(C(O)=NC3=NC2CCCCC2)c2cccc(F)c2)cc1 |w:20.22,c:18|
Structure
BACE Substrate Peptide
Name:BACE Substrate Peptide
Synonyms:BACE-1 Substrate Peptide
Type:Peptide
Mol. Mass.:1557.77
Organism:n/a
Description:It is a coumarin (CM)-labeled peptide containing an optimized cleavage sequence NFEV.
Residue:12
Sequence:
CM-REVNFEVEFR