| Assay Method Information | |
| | Fluorescence Resonance Energy Transfer Assay (FRET) |
| Description: | The assay was performed in a 96-well plate, each well contained substrate peptide, LF, and the test compound. The C-terminally fluorophore of substrate peptide was 7-hydroxy-4-methyl-3-acetylcoumarinyl, and the acceptor chromophore was N-({4-[(7-nitro-2, 1, 3-benzoxadiazol-4-yl) amino] phenyl} acetyl. Cleavage of the substrate by LF released the fluorophore and full fluorescence was restored. The reaction was examined using a fluorescent plate reader at excitation/355 nm and emission/460 nm. |
| Affinity data for this assay | |
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