| Assay Method Information | |
| | Measurement of FBSAChE/EqBuChE Inhibitory Activity |
| Description: | Inhibition of enzyme activity was measured over a substrate concentration range of 0.01-30 mM and at least six inhibitor concentrations to determine competitive and noncompetitive inhibitors. Plots of initial velocities versus substrate concentrations at a series of inhibitor concentrations were analyzed by nonlinear least-squares methods to determine the values of Km and Vmax. Nonlinear regression analysis of the plots of Vmax/Km values versus [THA-An] was used for the determination of Ki value |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |