| Assay Method Information | |
| | 3CL Protease Enzyme Assays |
| Description: | The effects of compound on enzyme activity were measured by using peptide cleavage assay. Cleavage products were resolved and analyzed with a reverse-phase HPLC. Quantification of peak areas was used to determine the extent of substrate conversion. Ki was calculated using the equation of Cheng and Prusoff. |
| Affinity data for this assay | |
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