| Assay Method Information | |
| | DPPIV Inhibition Assay |
| Description: | The DPP4 activity resulted in the formation of the fluorescent product amidomethylcoumarin (AMC), which was monitored by excitation at 370 nm and measurement of fluorescent emission at 450 nm. To assess the inhibitory potential of the compounds, IC50 values were determined from at least two independent measurements carried out as triplicates. |
| Affinity data for this assay | |
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