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Assay Method Information

Assay Name:  Fluorogenic Assay
Description:  DPP-IV inhibitors were measured for their ability to inhibit DPP-IV mediated cleavage of Ala-Pro-7-amido-4-trifluoromethylcoumarin in a fluorogenic assay. The compounds were measured in triplicate at 5 to 7 concentrations in the range of 100 uM to 100 pM. IC50 values were calculated with a nonlinear best-fit regression model. All assays were calibrated with NVP-DPP728 as internal standard inhibitor.
Affinity data for this assay
 

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Last update November 1, 2007
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