| Assay Method Information | |
| | CDK Inhibition Assay |
| Description: | In vitro kinase assay using purified enzyme, was incubated at 30 °C with substrate, and test compounds in the presence of 12.5 uM ATP/ [gamma-32P] ATP. 32P incorporation into histone H1 by using a Phospho-Image plate read on an FLA3000 plate reader, and the resulting images were analyzed using ImageGauge. IC50 values were determined by least-squares fitting of the data to the Hill equation. |
| Affinity data for this assay | |
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