BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  ADK Inhibition Assay
Description:  ADK activity was monitored by a radiochemical assay, which measures the conversion of radioactive Ado ([U-14C] adenosine or [2-3H] adenosine) to AMP in the presence of ATP and MgCl2. The radioactivity incorporated was directly proportional to the conversion rate. The reaction was terminated by aliquoting the reaction mixture onto DE-81 anion exchange filter disks. The filter disks were then dried, washed, and counted. Bound radioactivity was determined by standard scintillation spectrometry at an efficiency of 55%.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.