Assay Method Information | |
| Binding Assay |
Description: | Stable and optimal assay conditions were determined by cross-titrating GST-AKAP18δ and biotinylated PLB using 10 μg/ml glutathione acceptor beads and 10 μg/ml streptavidin donor beads in an AlphaScreen assay. The excitation wavelength was 680 nm, with the emission wavelength being 520-560 nm. Signal intensity in each well was registered and the optimal concentration to use was chosen to be the concentration prior to the peak of the signal. |
Affinity data for this assay | |
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