| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | The IC50s were determined by incubating each test compound with activated p38-alpha in a 96-well plate that had been pre-treated with ATF-2-GST to allow adherence of this substrate to the bottom of the well. After a 60-min incubation, the amount of phosphorylated ATF-2 produced was assessed by an ELISA-type readout. |
| Affinity data for this assay | |
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