| Assay Method Information | |
| | Determination of Inhibitor Potency and Selectivity |
| Description: | Ki values for compounds were calculated by incubation of each enzyme with its substrate and various compound concentrations. Absorbance was read at 405 nM following preincubation (background, time zero measurement) and following the 30 min incubation with substrate using a microplate reader. Background values were subtracted from the final absorbance values. Percentage inhibition was calculated and plotted against compound concentration to generate IC50 values. The enzymatic Ki was calculated from the known Km of each substrate, using the equation: Ki = IC50 / ((1 + ([S]/Km)). |
| Affinity data for this assay | |
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