| Assay Method Information | |
| | DPP Inhibition Assay |
| Description: | Compounds were tested for their ability to inhibit DPP enzymes mediated cleavage of Ala-Pro-7-amido-4-trifluoromethylcoumarin in a fluorogenic assay. The reaction was monitored by excitation at 400 nm and measurement of fluorescent emission at 505 nm for 5-10 minutes or an end-point was measured after 10 minutes. IC50 values were calculated from the enzyme progress curves using standard mathematical models. |
| Affinity data for this assay | |
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