| Assay Method Information | |
| | Chk1 Enzymatic Assay |
| Description: | In vitro Chk1 enzymatic assay using purified enzyme, was incubated with substrate, and test compounds in the presence of 10 uM ATP/ [gamma-32P] ATP. 32P incorporation was measured with a scintillation counter. IC50 values were determined by a least-squares fit to the equation, CPM = Vmax X(1 - ([I]/(IC50 + [I]))) + nonspecific binding. Ki,app = IC50/(1 + [ATP]/Km). |
| Affinity data for this assay | |
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