Assay Method Information

Assay Name:  Jurkat J6 Scintillation Proximity Assay
Description:  J6 cells (1 million cells/well) were allowed to coat wheat germ agglutinin coated SPA beads (Amersham, 1 mg/well) in assay buffer. Tritiated 3H Standard Compound A and test compounds were dissolved in an appropriate solvent and diluted in assay buffer. Data are presented as mean pKi.
Affinity data for this assay
 

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