Assay Method Information

Assay Name:  Cell Adhesion Assay (alpha4beta1 Mediated Adhesion/VCAM-1)
Description:  The VLA-4 expressing U937 cells were plated in 96-well microtiter plates, precoated with soluble human recombinant VCAM-1, in the presence of test compounds or vehicle (DMSO, 1% final concentration). Plates were then incubated at room temperature for 45 min. After washings, adherent cells were quantified by fluorescence measurement and expressed in percentage of total cells. Adhesion inhibition was calculated for each test compound concentration run in triplicate. IC50 were evaluated from three independent titration curves and expressed as mean value.
Affinity data for this assay
 

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