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Assay Method Information

Assay Name:  Competitive Fluorescent-binding Assay
Description:  AVPC-badan can be displaced from the binding pocket of the protein by test compounds. As the dye is displaced from the binding pocket, the emission is reduced and resembles that of the AVPC-badan dye in solution alone. The AUC for the emission spectra of AVPC-badan alone, AVPC-badan, and for the Smac mimic bound to BIR3-XIAP are used to calculate a relative Kd for the Smac mimic. The relative Kd is then converted to an absolute Kd using the Kd value for AVPC-badan, which was determined in a titration assay.
Affinity data for this assay
 

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Last update November 1, 2007
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