| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | MetAP2 activity was monitored by measuring the initial velocity of turnover of the artificial substrate Met-AMC. Assays were performed in 96-well microtiter plates using recombinant N-terminal truncated human methionine aminopeptidase, with Co2+ as a cofactor. In a typical 96-well plate assay, the increase in the fluorescence emission (excitation at 360 nm, emission at 460 nm) ) in each well was monitored continuously using a Cytofluor series 4000 multiwell plate reader and used to calculate the initial velocity of hMetAP2. Ki data was fitted to the Dixon competitive inhibition equation using Grafit (Erithacus software). |
| Affinity data for this assay | |
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