| Assay Method Information | |
| | cFMs Autophosphorylation Fluorescence Polarization End Point Assay. |
| Description: | The full-length cFMS cytoplasmic domain (FMS.538-972.6His) was incubated with compound in reaction buffer. Control reactions were run in each plate. Assay buffer was substituted for the compound in positive and negative control wells. The fluorescence polarization was measured after 30 min of incubation. Under these conditions, the polarization values for positive and negative controls were around 300 mP and 150 mP, respectively, and were used to define the 100 and 0% inhibition of the FMS reaction. The IC50 values reported are the averages of three independent measurements. |
| Affinity data for this assay | |
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