Assay Method Information | |
| HMG-CoA Reductase In Vitro Assay |
Description: | Assay for HMG-CoA reductase was based on the conversion of isotopically labeled HMG-CoA to mevalonic acid using rat liver microsomes as enzyme source. The product, [14C]-mevalonic acid was lactonized and separated from excess substrate via an anion ion exchange resin column, and radioactivity was counted on a scintillation counter. |
Affinity data for this assay | |
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