| Assay Method Information | |
| | Kinesin ATPase In Vitro Assay |
| Description: | The kinesin motor domain is incubated with microtubules, 1 mM ATP (1: 1 MgCl2 : Na-ATP), and compound at 23°C in buffer. After reaction was terminated, free ph osphate from the ATP hydrolysis reaction is measured via a quinaldine red/ammoni um molybdate assay. The absorbance of the phospho-molybdate complex is measured Human poly-histidine tagged KSP motor domain was cloned, and protein was expressed in E. coli. |
| Affinity data for this assay | |
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