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Assay Method Information

Assay Name:  Fluorogenic Assay for Detection of FASTE Inhibition
Description:  The reaction mixture consisted of FAS thioesterase domain (FASTE) in buffer, which was preincubated with test compounds for 30 min. The reaction was initiated by addition of substrate 4-MUH. The resulting fluorescence from liberated 4-methylumbelliferone was measured every 5 min at 350|450 nm for 40-60 min. 4-MUH incubated without enzyme served as a background control. Results are the average of triplicate data points.
Affinity data for this assay
 

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Last update November 1, 2007
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