Assay Method Information

Assay Name:  In Vitro BRAF Kinase Assay
Description:  BRAF kinase activity was quantified using an ELISA-based MEK phosphorylation assay. IC50 values were derived from the sigmoidal dose-response curve fitting by GraphPad Prism 4.0. Ki was calculated from the Cheng-Prusoff equation.
Affinity data for this assay
 

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