| Assay Method Information | |
| | Fluorescence Polarization Assay |
| Description: | Fluorescence polarization was measured on an Ultra plate reader (Tecan) at excitation and emission wavelengths of 485 and 530 nm, respectively. The equilibrium binding curves were drawn by plotting polarization units as a function of recombinant XIAP concentration. All experiments were performed in black, flat-bottomed, 96-well microplates. The Smac mimetics were evaluated for their ability to displace the fluorescent probe from recombinant protein. The Ki values were derived from IC50s according to Cheng-Prusoff equation. |
| Affinity data for this assay | |
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