Assay Method Information

Assay Name:  Aurora Kinase Assay
Description:  Assays for Aurora kinase was performed in a DELFIA format. Aurora enzyme was incubated with test compound and cross-tide substrate in the reaction buffer. After incubation, the reaction mixtures were transferred to a neutravidin-coated plate, and phosphorylated peptide was quantified by means of a phosphor-specific antibody (Cell signaling Technology) and a europium labeled secondary antibody (Perkin-Elmer) using time-resolved fluorescence (excitation, 337 nm; emission, 620 nm).
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail