| Assay Method Information | |
| | Fluorescence Resonance Energy Transfer (FRET) Ligand Sensing Assay (LiSA) |
| Description: | The LXR LiSA measures the ligand-dependent recruitment of a 25 amino acid fragment of the steroid receptor coactivator 1 (SRC1) to the ligand-binding domain of the receptor. The biotinylated protein was incubated with streptavidin-AlloPhycoCyanin, biotinylated SRC-1 peptide, and streptavidin-labeled europium. The fluorescence resonance energy transfer was then counted on a Wallac Victor fluorescent plate reader in a time-resolved mode. The relative fluorescence was measured at 665 nm. |
| Affinity data for this assay | |
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