Assay Method Information | |
| Fluorescence Polarization-Based Binding Displacement Kinase Assay |
Description: | The assay is based on the kinase-bound labeled fluorescent probe can be displaced by competitive inhibitors, which can be registered by measurement of the resulting change in fluorescence properties of the solution. The binding curves were fitted using GraphPad Prism version 4.03 using sigmoidal dose-response variable slope regression functions. The dissociation/displacement constants Kd were calculated using online tool for fluorescence-based competitive binding assays. |
Affinity data for this assay | |
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