| Assay Method Information | |
| | MAPKKide Assay |
| Description: | The fluorescence peptide cleavage assay was performed in a 96-well plate in which each reaction mixture contained MAPKKide, LF (List Biological Laboratories), and the screening compounds. Kinetics of the peptide cleavage was examined for 30 min by using a fluorescence plate reader at excitation and emission wavelengths of 485 and 535 nm, respectively, and IC50 values were obtained by dose-response measurements. For selected compounds, Lineweaver-Burk analysis was also carried out to verify that the compounds are competitive against the substrate. |
| Affinity data for this assay | |
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