Assay Method Information

Assay Name:  NOS Enzyme Inhibition Assay
Description:  Nitric oxide formation from NOS was monitored by the hemoglobin capture assay. The assay was initiated by addition of enzyme and was monitored at 401 nm on a Perkin-Elmer Lambda 10 UV-vis spectrophotometer. The apparent Ki values were obtained by measuring percent inhibition in the presence of 10 uM L-arginine with at least four different concentrations of inhibitor. The IC50 values were determined by linear regression analysis of the percent inhibition data. Inhibition constants Ki were derived from the equation, Ki = IC50 / (1+ [S]/Km).
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail