| Assay Method Information | |
| | Radioligand Binding Assay (Ki) and cAMP Accumulation Assay (EC50) |
| Description: | Radioligand binding studies were performed in buffer contains test compound, [3H]-GR113808, and receptor C6 glial cell membrane preparation. Nonspecific binding was determined with 10 uM GR113808. After incubation, the reaction was terminated by filtration through Whatman GF/B. Radioactivity was measured using a Beckman model LS6500C liquid scintillation counter. Binding data are expressed as a percentage of displacement for the screening of the library compounds. For selected compounds, Ki were analyzed by computer-assisted nonlinear regression analysis (Prism, Graphpad Software, San Diego, CA). EC50 values correspond to the concentration of agonists required to obtain half maximal stimulation of adenylyl cyclase. The maximum response produced by each molecule was normalized to the 5-HT induced. The data are the results of two or three independent determinations performed in triplicate. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |