| Assay Method Information | |
| | ELISA assay |
| Description: | A homogeneous in vitro assay based on a LANCE format was first used for high-throughput screening. Those compounds that showed the largest differential inhibition of PDK1 and SGK1 were subjected to an ELISA assay in which direct inhibition of SGK1 phosphorylation was measured by using anti-phospho-SGK1 antibody. ELISA IC50s were determined by Microsoft Excel data analysis wizard model 63 with the luminescent signal from wells without compound treatment as 100%. |
| Affinity data for this assay | |
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