Assay Method Information | |
| Enzyme Inhibition Assay |
Description: | Phosphorylase activity was measured in the direction of glycogen synthesis by the release of orthophosphate from Glc-1-P. The enzyme was assayed in glycogen with various concentrations of Glc-1-P, AMP and inhibitors. Inorganic phosphate released in the phosphorylase reaction was determined. Ki values, at each concentration of inhibitor, were then determined by plotting Km(app) versus inhibitor concentration. |
Affinity data for this assay | |
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