Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  Phosphorylase activity was measured in the direction of glycogen synthesis by the release of orthophosphate from Glc-1-P. The enzyme was assayed in glycogen with various concentrations of Glc-1-P, AMP and inhibitors. Inorganic phosphate released in the phosphorylase reaction was determined. Ki values, at each concentration of inhibitor, were then determined by plotting Km(app) versus inhibitor concentration.
Affinity data for this assay
 

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