| Assay Method Information | |
| | Pim Kinase Assay |
| Description: | In 384-well v-bottom polypropylene plates, compound (2% DMSO) was mixed with Pim kinase and peptide substrate, followed by immediate initiation with gamma-[33P] ATP. Reactions were quenched after 1 h by the addition of stop buffer. Eighty microliters of the stopped reactions were transferred to 384-well streptavidin-coated plates (Flash-Plate Plus, Perkin-Elmer), incubated 30 min at room temperature and washed three times. Radioactivity was counted on a TopCount Scintillation Plate Reader (Packard). |
| Affinity data for this assay | |
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