| Assay Method Information | |
| | Absorbance Microorganism Dose Response to Identify Inhibitors of Vibrio harveyi |
| Description: | Keywords: Quorum Sensing, LuxPQ receptor, AI-2 Assay Overview: The overall aim of the project is to identify inhibitors of the quorum sensing pathway in Vibrio harveyi. Previously, a primary assay was completed using a strain containing only the AI-2 quorum sensing system intact. The primary screen could potentially identify inhibitors of the AI-2 synthase LuxS, the AI-2 receptor LuxPQ, or other downstream elements of the pathway. Subsequent secondary assays and counter screens, such as the current assay, will be used to determine the mode of action of compounds identified in the primary screen. This current assay uses the strain MM32, a V harveyi line which is null for LuxS, the AI-2 synthetic enzyme, and AI-1 based quorum sensing but which maintains the AI-2 receptor system LuxPQ. Consequently, this assay uses exogenously supplied AI-2 to determine if compounds antagonize LuxPQ. Expected Outcome: Compounds which antagonize LuxPQ will inhibit the luminescent signal caused by |
| Affinity data for this assay | |
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