Assay Method Information

Assay Name:  SAR Confirmatory Dose Response LIBS Assay for Allosteric Ligands of the VLA-4 Integrin
Description:  University of New Mexico Assay Overview: Assay Support: 1 R01 HL081062-01 Project Title: HTS for Identification of VLA-4 Allosteric Modulators (MLPCN) PI: Larry Sklar Screening Center / PI: UNM Center for Molecular Discovery / Larry Sklar Chemistry Center / PI: Vanderbilt Specialized Chemistry Center / Jeff Aube Assay Implementation: Peter Simons, Yang Wu, Susan Young, Terry Foutz, Stephanie Sedillo, Anna Waller, Annette Evangelisti, Mark Carter, Oleg Ursu, Cristian Bologa Assay Background and Significance: We have developed a novel ligand induced binding site (LIBS) mAb assay for integrin HTS using phycoerythrin (PE) labeled HUTS-21 mAb. The novel assay is a result of an R01 (HL081062) which has taken advantage of compounds identified through an earlier project X01MH077638 (entitled "MLSCN Assay for Allosteric Regulators of the VLA-4 Integrin") that screened a portion of the MLSMR for allosteric regulators of VLA-4 (AIDs 528, 529). The approach described here uses an assay
Affinity data for this assay
 

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