Assay Method Information | |
| Kinase Inhibition Assay |
Description: | IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labeled ATP to a protein or peptide substrate. Use of 32P-labeled ATP allows this transfer to be monitored by scintillation counting or by autoradiography of the substrate |
Affinity data for this assay | |
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