| Assay Method Information | |
| | DAPK1 Electrophoretic Mobility Shift Assay |
| Description: | The reaction was started by adding a mix of ATP and Peptide 38 to assay plates containing DAPK1. Prior to mixing, all reagents are diluted in assay buffer (100 mM HEPES (pH 7.5), 5 mM MgCl2, 0.05% CHAPS, 0.5 mM DTT and 0.1 mg/ml IgG, 10 µM Na-Orthovanadate). The 20 µL reaction solution was incubated 60 min at room temperature and then stopped by addition of 5 µL EDTA to a final concentration of 10 mM. |
| Affinity data for this assay | |
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