| Assay Method Information | |
| | Alpha-glucosidase Assay |
| Description: | Typically, α-glucosidase activity was assayed in 50 mM phosphate buffer (pH 6.8) containing 5% v/v DMSO and PNP glycoside was used as a substrate. The inhibitors were pre-incubated with enzyme at 37 °C for 0.5 h. The substrate was then added and the enzymatic reaction was carried out at 37 °C for 60 min. The reaction was monitored spectrophotometrically by measuring the absorbance at 400 nm. |
| Affinity data for this assay | |
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