Assay Method Information | |
| Fluorogenic ACC Substrate Assay |
Description: | Reactions were carried out in solutions (50 µL) containing PgaB (10 µM) in HEPES (100 mM, pH 7.5), and varying concentrations of ACC (0.05-10 mM) at 37 °C in a 96-well plate. Before adding the substrate, the reaction solution was pre-incubated at 37 °C for 10 min. |
Affinity data for this assay | |
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