| Assay Method Information | |
| | Carbonic Anhydrase (bCAII) Inhibition Assay |
| Description: | This was assay performed using buffer containing Tris and HEPES at the pH of 7.2-7.9 and a total concentration of 20 mM. 96-well plates were filled with 140 µL of Tris-HEPES solution, 20 µL of bovine erythrocyte carbonic anhydrase II solution (0.1-0.2 mg/2000 mL), and 20 µL of 0.7 mM para-nitrophenyl acetate (diluted in ethanol). Test compounds were incubated at 25-28 °C for 15 min prior to addition of para-nitrophenyl acetate, which starts the reaction. The reaction was allowed to taking place for 30 min at 25-28 °C. All compounds tested in triplicate at different concentration. The amount of product that forms during thereaction monitored at 400 nm after 30 min using SPECTRA-max 340 spectrophotometer, Molecular Devices (USA). |
| Affinity data for this assay | |
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