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Assay Method Information

Assay Name:  AChE/BuChE Inhibition Assay
Description:  For AChE or BuChE inhibition assays, a reaction mixture (100 mL) containing acetylthiocholine iodide (1 mmol/L, 30 mL) or butyrylthiocholine iodide (1 mmol/L, 30 mL), phosphate-buffered solution (0.1 mmol/L, pH = 8.0, 40 mL), 0.5 U/mL EeAChE or 25% serum (10 mL) and different concentrations of test compounds (20 mL) was incubated at 37 C for 15 min. Then 5,5'-dithiobis-2-nitrobenzoic acid (DTNB, 0.2%, 30 mL) was added to produce the yellow anion of 5-thio-2-nitro-benzoic acid. Changes in absorbance were detected at 412 nm for EeAChE and 405 nm for ratBuChE in a Varioskan Flash Multimode Reader (Thermo Scientific).
Affinity data for this assay
 

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Last update November 1, 2007
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