| Assay Method Information | |
| | β-Glucuronidase Inhibition Assay |
| Description: | β-Glucuronidase inhibitory activity was evaluated by a biochemical assay, based on the measurement of the absorbance of p-nitrophenol at 405 nm, produced from the enzymatic hydrolysis of chromogenic substrate [Choudhary et al., Steroids, 74:1040-1044; Khan et al., Chem. Pharm. Bull., 50:1443-1446]. The reaction mixture, consisting of 5 μL of test compound solution, 185 μL of 0.1 M acetate buffer (pH 7.0) and 10 μL β-glucuronidase solution, was kept for 30 min at 37°C. The total reaction volume being 250 μL. 50 μL of p-nitrophenyl-β-D-glucuronide was added to each plate. A multiplate reader was used to read the absorbance at 405 nm. |
| Affinity data for this assay | |
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