Assay Method Information

Assay Name:  hCA Activity Assay
Description:  Esterase activities of hCA I and hCA II isoenzymes, eluted from affinity column,were determined by hydrolysis of p-nitrophenylacetate. The change of absorbance was determined at 348 nm after 3 min [Wilbur et al., J. Biol. Chem., 176:147-154].
Affinity data for this assay
 

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