| Assay Method Information | |
| | In Vitro PX Assay |
| Description: | 293 cells stably transfected with Nav 1.5 were recorded in whole cell voltage clamp mode with the PatchXpress automated electrophysiology system according the manufacturer's specifications (Molecular Devices, LLC, Sunnyvale, Calif.). Cells were held at a holding potential of 50 mV to inactivate sodium channels. To elicit sodium currents the voltage was changed to 120 mV to recover a portion of the channels, followed by delivery of test pulses of 20 msec duration to 0 mV, at 0.1 Hz. A single concentration of test compound was applied to cells for a duration of 5 minutes. |
| Affinity data for this assay | |
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