| Assay Method Information | |
| | TrkA Enzyme Assay |
| Description: | TrkA kinase activity was determined by measuring the kinase ability to phosphorylate synthetic tyrosine residues within a generic polypeptide substrate using amplified luminescent proximity assay (Alphascreen) technology (Perkin-Elmer, 940 Winter Street, Waltham, MA). The reaction products were detected with the addition of streptavidin coated donor beads and phosphotyrosine-specific antibodies coated acceptor beads. Plates were read using the EnVision Multilabel plate reader after an overnight incubation at room temperature. |
| Affinity data for this assay | |
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