| Assay Method Information | |
| | Electrophysiology Assay |
| Description: | After establishing the whole-cell configuration in voltage clamp mode, voltage protocols were run to establish the 1) test potential, 2) holding potential, and 3) the conditioning potential for each cell. After establishing the whole-cell configuration in voltage clamp mode, a standard I-V protocol was run to determine the potential at which the maximal current (Imax) is elicited. This potential was the test potential (Vt). To determine a conditioning potential at which 100% of channels were in the inactivated state, a standard steady-state inactivation (SSIN) protocol was run using a series of fifteen 100 ms-long depolarizing prepulses, incrementing in 10 mV steps, immediately followed by a 5 ms testing pulse, Vt, to Vmax. This protocol also permitted determination of the holding potential at which all channels are in the resting state. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |