Assay Method Information

Assay Name:  Inhibition Assay (piHA-Dm)
Description:  After kinetic analysis with HPA, piHA-Dm was tested as an inhibitor of ten additional enzymes. The conditions are listed as follows: Agrobacterium faecalis β-glucosidase (2.5 × 10^-4 mg/mL enzyme, 50 mM sodium phosphate pH 7, 4 mM PNP β-gal); Bos taurus β-galactosidase (6.9 × 10^-2 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM PNP β-gal); S. cerevisiae α-glucosidase (5 × 10^-3 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM α-glc); Human maltase-glucoamylase (8.1 × 10^-2 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM α-glc); Mouse maltase-glucoamylase (8.1 × 10^-2 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM α-glc); Butyrivibrio fibrisolvens α-amylase (1.7 × 10^-4 mg/mL enzyme, 50 mM sodium phosphate 100 mM NaCl pH 7, 1 mM CNPG3); Aspergillus oryzae α-amylase (1.9 × 10^-4 mg/mL enzyme, 50 mM sodium phosphate 100 mM NaCl pH 7, 1 mM CNPG3); Porcine pancreatic α-amylase (6.7 × 10^-3 mg/mL enzyme, 50 mM sodium phosphate 100 mM N
Affinity data for this assay
 

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