| Assay Method Information | |
| | Chemiluminescent Assay |
| Description: | PARP2 and PARP3 enzymatic assays were conducted using commercial PARP-2/PARP-3 Chemiluminescent Assay Kit (BPS Biosciences) and the protocols with the kits. Briefly, histones were coated in a high binding plate first, and incubated with PARP-2 or PARP-3, and increasing concentrations of compounds for 0.5 h. Then, biotinylated NAD and activated DNA were added to the wells. The biotinylated PARsylation product was measured by adding streptavidin-HRP and HRP substrates which produce chemiluminescence. |
| Affinity data for this assay | |
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