Assay Method Information | |
| Rv3378c Inhibition Assay |
Description: | For inhibition assay of Rv3378c, using tuberculosinyl diphosphate and adenosine as substrates, a mixture of 100 µM TPP, 100 µM adenosine,75 µg/mL Rv3378c, and inhibitors in the assay buffer (25 mM Tris-HCl, 1 mM MgCl2, 0.01% Triton-X-100) was incubated for 2 h at 37 °C. |
Affinity data for this assay | |
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