Abstract
A clinical strain of Klebsiella pneumoniae was found to possess the chromosomal gene bla(SHV-56), encoding a new inhibitor-resistant beta-lactamase with a pI of 7.6. SHV-56 is derived from SHV-11 by the single substitution K234R. This mutation therefore evidences a new critical site for inhibitor resistance among SHV enzymes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Amino Acid Substitution
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Base Sequence
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Female
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Genes, Bacterial
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Humans
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Isoelectric Point
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Kinetics
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Klebsiella Infections / drug therapy
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Klebsiella Infections / microbiology
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Molecular Sequence Data
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Mutagenesis, Insertional
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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beta-Lactam Resistance / genetics
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beta-Lactamases / chemistry*
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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DNA Primers
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DNA, Bacterial
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Recombinant Proteins
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beta-lactamase SHV-11
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beta-Lactamases